Enzymes assays

fdssucell application
 

FDSS is an “Add & Read” kinetic plate reader in Fluorescence and Luminescence, and suitable for reading the kinetics of the living cells which those response, calcium flux, mobilization, membrane potential changes, which is very fast and need to monitor its kinetic property. GPCR and Ion Channels and Transporter are the most highly used application field for FDSS kinetic reader monitoring the calcium transient and membrane potential changes of the living cells.

However, some creative work done at also in the Enzyme field, and FDSS is now not limited to the living cell. Normally, the enzyme assay does not require an “Add & Read” kinetic plate reader, since its reaction going on is very slow, so one sampling point per each 30 minutes or hour would be sufficient. But some enzyme reacts very fast which here requires our “Add and Read” kinetic plate reader system.

It is important to measure enzymatic activity during initial velocity phase of the reaction. However, continuous monitoring of assay signals has not always been practical in the past. The FDSS provides a solution in HTS environment, particularly for fast reactions.

The example shown here, are all done by the FDSS7000EX or the former high-end model called FDSS6000, the high- end system. FDSS7000EX has inherited all the optic design and fully compatible with FDSS6000. These assays are making use of UV excitation available in FDSS7000EX.

Related scientific material

Publications
Authors Title Source
Mori T, Itami S, Yanagi T, Tatara Y, Takamiya M, Uchida T. Use of a real-time fluorescence monitoring system for high-throughput screening for prolyl isomerase inhibitors J Biomol Screen. 2009 Apr;14(4):419-24.
Erica VanderPorten, Lauren Frick, Rebecca Turincio, Peter Thana, William LaMarr, Yichin Liu Label-free high-throughput assays to screen and characterize novel lactate dehydrogenase inhibitors Volume 441, Issue 2, 15 October 2013, Pages 115–122
Application Notes
App. Notes N°. Title
FDSS Application Note N°. 19 FDSS Application Note No.19 Fast kinetics enzyme assay High-throughput screening of prolyl isomerase (Pin1) inhibitors [0.2MB/PDF]
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