GPCR

fdssucell application
 

FDSS is a kinetic plate reader in Fluorescence and Luminescence, and suitable for reading the kinetics of the living cells which those response, calcium flux, mobilization for example, is very fast and need to monitor its kinetic property. GPCR is one of the most highly used application field for FDSS kinetic reader monitoring the calcium transient of the living cells. From the basic fluorescence dye such like Fluo-4, Fura-2 and to more various type of fluorescence dye such like FRET, and Flash Luminescence (Aequorine etc.,) reading technology can be used in our Kinetic reading platform.

FDSS7000EX is a lamp&filter base High-end system, capable of 1536 cell based assay with multiple washing ability and 2 dispenser head setup for the sticky compounds. It has more flexibility in having multiple of reading settings. Ratiometric Fura-2, Single wavelength Fluo-3 type Calcium flux, mobilization reading are possible. Some dye which requires FRET can also be done by using the motorized emission filter wheel option. By making use of its flexibility, maximum 2 excitation/2emission reading is possible with this system. From basic Calcium influx of the living cells, and to latest trend using human iPSC derived cardiomyocyte calcium transient reading which requires very high speed recording making use of our sensor camera capability, can be done in this system.

FDSS/μCELL is a more smaller foot print, simple version of FDSS for 96/384 kinetic plate reading. Difference from FDSS7000EX is that using LED as a light source for more stability and robustness, and Fluo-3 single wavelength excitation type of dye can be used in this system. This system also have an option of motorized emission filter wheel and FRET type of assay is possible. LED line up of Blue excitation, Green Excitation, C/Y FRET excitation, VSP FRET excitation are available.

Both system can be equipped with our high sensitive, high speed EM-CCD camera which enables Flash Luminescence and Ultra high speed recording of sampling, around 10ms interval as the shortest sampling interval, maximum 100Hz, reading is possible.

Related scientific material

Publications
Authors Title Source
Le Poul E, Hisada S, Mizuguchi Y, Dupriez VJ, Burgeon E, Detheux M. Adaptation of aequorin functional assay to high throughput screening J Biomol Screen. 2002 Feb;7(1):57-65.
Inbe H, Watanabe S, Miyawaki M, Tanabe E, Encinas JA. Identification and characterization of a cell-surface receptor, P2Y15, for AMP and adenosine J Biol Chem. 2004 May 7;279(19):19790-9. Epub 2004 Mar 4.
McNamara CR, Mandel-Brehm J, Bautista DM, Siemens J, Deranian KL, Zhao M, Hayward NJ, Chong JA, Julius D, Moran MM, Fanger CM. TRPA1 mediates formalin-induced pain http://www.ncbi.nlm.nih.gov/pubmed/17686976
Cassutt KJ, Orsini MJ, Abousleiman M, Colone D, Tang W. Identifying nonselective hits from a homogeneous calcium assay screen J Biomol Screen. 2007 Mar;12(2):285-7. Epub 2007 Feb 8.
Wise H, Abel PW, Cawkill D. Use of reduced temperature cell pausing to enhance flexibility of cell-based assays J Biomol Screen. 2009 Jul;14(6):716-22. Epub 2009 May 21.
Lin Tian, S. Andrews Hires, Tianyi Mao, Daniel Huber, M. Eugenia Chiappe, Sreekanth H. Chalasani, Leopoldo Petreanu, Jasper Akerboom, Sean A. McKinney, Eric R. Schreiter, Cornelia I. Bargmann, Vivek Jayaraman, Karel Svoboda and Loren L. Looger Imaging neural activity in worms, flies and mice with improved GCaMP calcium indicators Nature Methods 6, 875 - 881 (2009), online: 8 November 2009 | doi:10.1038/nmeth.1398
Pantel J, Williams SY, Mi D, Sebag J, Corbin JD, Weaver CD, Cone RD. Development of a high throughput screen for allosteric modulators of melanocortin-4 receptor signaling using a real time cAMP assay Eur J Pharmacol. 2011 Jun 11;660(1):139-47. Epub 2011 Feb 4.
Jasper Akerboom, Tsai-Wen Chen, Trevor J. Wardill, Lin Tian, Jonathan S. Marvin, Sevinç Mutlu, Nicole Carreras Calderón, Federico Esposti, Bart G. Borghuis, Xiaonan Richard Sun, Andrew Gordus, Michael B. Orger, Ruben Portugues, Florian Engert, John J. Macklin, Alessandro Filosa, Aman Aggarwal, Rex A. Kerr, Ryousuke Takagi, Sebastian Kracun, Eiji Shigetomi, Baljit S. Khakh, Herwig Baier, Leon Lagnado, Samuel S.-H. Wang, Cornelia I. Bargmann, Bruce E. Kimmel, Vivek Jayaraman, Karel Svoboda, Douglas S. Kim, Eric R. Schreiter and Loren L. Looger Optimization of a GCaMP Calcium Indicator for Neural Activity Imaging This article is freely available online through the J Neurosci Open Choice option. August 1, 2012
Olaposi I. Omotuyi, Jun Nagai & Hiroshi Ueda Lys39-Lysophosphatidate Carbonyl Oxygen Interaction Locks LPA1 N-terminal Cap to the Orthosteric Site and partners Arg124 During Receptor Activation Scientific Reports 5, Article number: 13343 (2015)doi:10.1038/srep13343
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