In-vitro toxicity assay

iPS derived cells drug discovery in FDSS

fdssucell application
 

Recently, studies of iPS cells (induced pluripotent stem cells) have made a huge impact in the drug discovery field. Currently, human iPS cell (hiPSC) derived various specific types of cells such as cardiomyocytes and neural cells are now widely available commercially, and the screening of chemical compounds for drug discovery using these hiPSC-derived cells is possible. Screening using hiPSC-derived cells is expected to provide more effective and easy way to evaluate the pharmacological and toxic effects of test compounds in cell-based assays.

HAMAMATSU FDSS series are capable for the measurement and analysis of calcium transient in hiPSC-derived cardiomyocytes.

iPSC derived Cardiomyocyte for Safety & Vascular [0.8MB / PDF
iPSC derived Neurons [4.4MB / PDF

 

Both FDSS7000EX and FDSS/μCELL can have the “Heater”, “High Speed Recording” and “Waveform Analysis” option, needed for iPSC cardiomyocyte calcium transient. “Heater” function becomes more and more important when handling iPSC, for its cell status and condition during the assay, especially affects the beating rate of cardiomyocyte. Another key technology and function to monitor the calcium transient is the “High Speed Recording” ability. Maximum around 100Hz sampling rate, shortest around 10ms interval, reading is possible with our high sensitive high speed EM-CCD camera. We also have an option of “Waveform analysis” software, to analyze the number of peak, beating rate, amplitude, rising slope, falling slope PWD from the measured calcium transient data.

Related scientific material

Publications
Authors Title Source
Stephane Bedut, Christine Seminatore Nole, Veronique Lamamy, Sarah Caignard, Jean Albert Boutin, Olivier Nosjean, Jean-Philippe Stephan, Francis Coge High-throughput drug profiling with voltage and calcium-sensitive fluorescent probes in human iPSC-derived cardiomyocytes American Journal of Physiology - Heart and Circulatory Physiology
Published 3 May 2016, Vol. no.
DOI:
10.1152/ajpheart.00793.2015
Haoyu Zeng, Maria I. Roman, Edward Lis, Armando Lagrutta, Frederick Sannajust, SALAR, Safety & Exploratory Pharmacology Department, Merck Research Laboratories, West Point, PA 19486, USA Use of FDSS/μCell imaging platform for preclinical cardiac electrophysiology safety screening of compounds in human induced pluripotent stem cell-derived cardiomyocytes Received 29 February 2016
Revised 6 May 2016
Accepted 18 May 2016
Available online 21 May 2016
http://dx.doi.org/10.1016/j.vascn.2016.05.009
Dinesh Puppala, Leon P. Collis, Sunny Z. Sun, Vinicius Bonato, Xian Chen, Blake Anson, Mathew Pletcher, Bernard Fermini and Sandra J. Engle Comparative Gene Expression Profiling in Human-Induced Pluripotent Stem Cell—Derived Cardiocytes and Human and Cynomolgus Heart Tissue Toxicol. Sci. (2013) 131 (1): 292-301.
First published online: Sept. 14, 2012
Related Scientific Materials from Users Meetings in the past
Year Title
2016 Triggering Calcium Responses in Various Human iPSC-derived Neural Cell Types
Giorgia Salvagiotto, CDI - FUJIFILM company, UK
2015 Axiogenesis growing portfolio on human iPSC-derived cells
2015 Measurement of Ca2 response in human iPSC-derived peripheral neurons by electric field stimulation in FDSS/µCell
2015 Electric Field Stimulation (EFS) of human iPSC-derived neuron using Hamamatsu FDSS/µCELL
 
iPSC Cardiomyocytes
Year Title
Workshop:
2016 Higher Throughput Calcium Transient Recording from hiPSC-derived Cor.4U Cardiomyocytes: Ready for CiPA Phase II Validation Study
Dr Ralf Kettenhofen, Axiogenesis
2016 Experimental parameters for an efficient recording of membrane potential and calcium activity from hIPS-derived cardiomyocytes in 96 wells
Stephane Bedut, VWR CATALYST/ICM, Paris, France
FDSS Users Meeting:
2016 Overview of FDSS application: With a focus on EFS assay
Natsumi Kato, Hamamatsu Photonics K.K., Hamamatsu, Japan
2016 Comprehensive in vitro Proarrythmia Assay (CiPA) Using Cor.4U Cardiomyocytes with the FDSS in a Calsium Transient Assay
Dr Ralf Kettenhofen, Axiogenesis, Cologne, Germany
2016 New Developments at Axiogenesis
Dr Ralf Kettenhofen, Axiogenesis, Cologne, Germany
2016 Multiparametricassessment of the effects of cardioactivecompounds on human iPSC-derived cardiomyocytes
Marijn Vlaming, Pluriomics B.V., Leiden, The Netherlands
2016 Novel assays to study drug effects in hiPSC-derived cells using the FDSS/μCell system
Marijn Vlaming, Pluriomics B.V., Leiden, The Netherlands
2015 In vitro predictive toxicity of GPCR modulators in human iPS-derived Cor.4U cardiomyocytes by Ca2+ transients detection
2015 Electric Field Stimulation (EFS) of iPSC-derived cardiomyocytes using Hamamatsu FDSS/µCell with fast data acquisition
2015 Safety toxicity with cardiomyocytes derived from induced human pluripotent stem cells on FDSS platform
2014 Detection of CA++Transients in iPS-derived Cardiomyocytes: an HTS-ready Method of Measuring Cardiomyocytes Function
2014 Electric Field Stimulation (EFS) of cardiomyocytes using Hamamatsu FDSS/µCell
2014 Electric Field Stimulation (EFS) of iPSC-derived cardiomyocytes using Hamamatsu FDSS/µCell with fast data acquisition
2014 High speed (over 100Hz) HTS using human iPSCs-derived cardiomyocytes on FDSS
2014 Human Cardiomyocytes Derived from Induced Pluripotent Stem Cells: High Throughput and High Content Assessment of Cardiac Toxicity and Drug Efficacy by Monitoring Cytosolic Free Calcium Transients
2014 HTS of Ca2+ Transients in Human iPS-derived Cardiomyocytes as a Predictive and Cost Effective Assay Early on in Drug Development.
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