Ion channels & transporter

fdssucell application
 

FDSS is a kinetic plate reader in Fluorescence and Luminescence, and suitable for reading the kinetics of the living cells which those response, calcium flux, mobilization, membrane potential changes, which is very fast and need to monitor its kinetic property. Ion Channels and Transporter are the most highly used application field for FDSS kinetic reader monitoring the calcium transient and membrane potential changes of the living cells. From the basic fluorescence dye such like Fluo-4, Fura-2, and various membrane potential dye, even sodium, potassium ion indicator dye as well as the calcium mobilization. FRET, and Flash Luminescence (Aequorine etc.,) reading technology can also be done in our Kinetic reading platform.

FDSS7000EX is a lamp&filter base High-end system, capable of 1536 cell based assay with multiple washing ability and 2 dispenser head setup for the sticky compounds. It has more flexibility in having multiple of reading settings. Ratiometric Fura-2, Single wavelength Fluo-3 type Calcium flux, mobilization membrane potential change reading are possible. Some dye which requires FRET can also be done by using the motorized emission filter wheel option. By making use of its flexibility, maximum 2 excitation/2emission reading is possible with this system.

From basic Calcium influx and membrane potential of the living cells, and to latest trend using human iPSC derived cardiomyocyte calcium transient and membrane potential reading which requires very high speed recording making use of our sensor camera capability, can be done in this system.

FDSS/μCELL is a more smaller foot print, simple version of FDSS for 96/384 kinetic plate reading. Difference from FDSS7000EX is that using LED as a light source for more stability and robustness, and Fluo-3 single wavelength excitation type of dye can be used in this system. This system also have an option of motorized emission filter wheel and FRET type of assay is possible. LED line up of Blue excitation, Green Excitation, C/Y FRET excitation, VSP FRET excitation are available.

Both system can be equipped with our high sensitive, high speed EM-CCD camera which enables Flash Luminescence and Ultra high speed recording of sampling in Fluorescence, around 10ms interval as the shortest sampling interval, maximum 100Hz, reading is possible.

Related scientific material

Publications
Authors Title Source
Kawamoto T, Kimura H, Kusumoto K, Fukumoto S, Shiraishi M, Watanabe T, Sawada H. Potent and selective inhibition of the human Na+/H+ exchanger isoform NHE1 by a novel aminoguanidine derivative T-162559 Eur J Pharmacol. 2001 May 18;420(1):1-8.
McNamara CR, Mandel-Brehm J, Bautista DM, Siemens J, Deranian KL, Zhao M, Hayward NJ, Chong JA, Julius D, Moran MM, Fanger CM. TRPA1 mediates formalin-induced pain Proc Natl Acad Sci U S A. 2007 Aug 14;104(33):13525-30. Epub 2007 Aug 8.
Ayala JE, Niswender CM, Luo Q, Banko JL, Conn PJ. Group III mGluR regulation of synaptic transmission at the SC-CA1 synapse is developmentally regulated Neuropharmacology. 2008 Apr;54(5):804-14. Epub 2007 Dec 24.
Niswender CM, Johnson KA, Luo Q, Ayala JE, Kim C, Conn PJ, Weaver CD. A novel assay of Gi/o-linked G protein-coupled receptor coupling to potassium channels provides new insights into the pharmacology of the group III metabotropic glutamate receptors Mol Pharmacol. 2008 Apr;73(4):1213-24. Epub 2008 Jan 2.
Kobayashi K, Nishizawa Y, Sawada K, Ogura H, Miyabe M. K(+)-channel openers suppress epileptiform activities induced by 4-aminopyridine in cultured rat hippocampal neurons J Pharmacol Sci. 2008 Dec;108(4):517-28. Epub 2008 Dec 11.
Delpire E, Days E, Lewis LM, Mi D, Kim K, Lindsley CW, Weaver CD. Small-molecule screen identifies inhibitors of the neuronal K-Cl cotransporter KCC2 Proc Natl Acad Sci U S A. 2009 Mar 31;106(13):5383-8. Epub 2009 Mar 11.
Kim Y, Kang S, Lee JY, Rhim H. High-throughput screening reveals a small-molecule inhibitor of the renal outer medullary potassium channel and Kir7.1 Mol Pharmacol. 2009 Nov;76(5):1094-103. Epub 2009 Aug 25.
Kim Y, Kang S, Lee JY, Rhim H. High throughput screening assay of alpha(1G) T-type Ca2+ channels and comparison with patch-clamp studies Comb Chem High Throughput Screen. 2009 Mar;12(3):296-302.
Titus SA, Beacham D, Shahane SA, Southall N, Xia M, Huang R, Hooten E, Zhao Y, Shou L, Austin CP, Zheng W. A new homogeneous high-throughput screening assay for profiling compound activity on the human ether-a-go-go-related gene channel Anal Biochem. 2009 Nov 1;394(1):30-8. Epub 2009 Jul 5.
Jinliang Sui, Shakira Cotard, Jennifer Andersen, Ping Zhu, Jane Staunton, Margaret Lee, and Stephen Lin Optimization of a Yellow Fluorescent Protein-Based Iodide Influx High-Throughput Screening Assay for Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) Modulators ASSAY and Drug Development Technologies. December 2010, 8(6): 656-668. doi:10.1089/adt.2010.0312.
C. David Weaver Triple-Addition Assay Protocols for Detecting and Characterizing Modulators of Seven-Transmembrane Receptors Current Protocols in Chemical Biology. 3:119–140.
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