FDSS7000EX Functional Drug Screening System
The FDSS7000EX is our high-end model capable of handling 1536-well assays and measuring both fluorescence and luminescence, and is equipped with a variety of functions such as multiple washing. Many kinds of suspended/adherent cells' real-time kinetic reactions can be measured and analyzed. Various optional parts such as FRET, robot connections, etc. are available. In addition, the FDSS7000EX is expandable for future upgrades.
Applications
- Intracellular calcium ion
- Membrane potential
- Ion channel
- Aequorin
- FRET
- Suspended cell
◇Measurement example: Intracellular calcium ion
Comparison of calcium mobilities using Aequorin, Fluo-3, and Fura-2 receptor-expressing cells.
Hardware
Dispenser
Can be equipped with various dispenser heads
- 96-, 384-, or 1536-tip dispenser heads are selectable. The tips are exchangeable.
- 384- and 1536-pintool heads are also supported.
- The main unit is equipped with an automatic tip loading feature.
- As standard, three types of compounds can be dispensed. If you use the back plate loading line, up to four types of compounds can be dispensed.
- The dispenser heads can be easily changed by the user.
Washing unit
Equipped with a variety of washing features
- Up to three solvents are supported, which enables washing with no carry over.
- Tips can be washed during measurement.
- An ultrasound washing feature is also available.
- Equipped with a wiping stage to dry tip ends.
Plate stacker unit
Plate stacker feature for 20 or 50 plates
- Using the plate stacker makes it possible to perform automatic measurements even without the use of a robot.
- You can select 20 plates or 50 plates.
- Stacker cassette method makes it easy to set plates.
- Can be equipped with a barcode reader.
Plate shaking features
Plate shaking provides stable response detection.
- Measurements can be performed while applying fine vibrations to the whole plate.
- Cells and reagents can be mixed effectively and quickly.
- This is also effective for assays that use suspended cells.
Fluorescence/luminescence sensor, Fluorescence optics
Supports fluorescence assays and luminescence assays with a single sensor
- This sensor has a fluorescence detection sensitivity that far exceeds that of conventional fluorescence sensors.
- Because the excitation light can be lowered, the cost of purchasing excitation light sources can be reduced.
- In addition to high sensitivity, the FDSS7000EX also has a high dynamic range.
- The fluorescence changer makes FRET measurements possible.
Various assays with xenon lamp & filter combination (multiple excitation and emission)
- Good wavelength separation by epi-illumination optics
- Fura-2 assay with dual excitation (340 nm & 380 nm)
- FRET (C/Y, Membrane potential) with emission filter changer
- 2 dichroic mirror and 4 emission filter slots for various assays
- White light xenon lamp with wattage options (150 W, 300 W, 600 W)
- 6 filter slots for excitation
- Uniform plate illumination
Specifications
| Type number | FDSS7000EX |
|---|---|
| Main unit dimensions | Approx. 1500 mm (W)×1030 mm (D)×1450 mm (H) |
| Main unit | Up to approx. 300 kg |
| Data analysis system dimensions | 600 mm (W)×700 mm (D)×1500 mm (H) |
| Data analysis system weight | Approx. 50 kg |
| Pump unit/Tank dimensions | 500 mm (W)×500 mm (D)×1200 mm (H) |
| Pump unit/Tank weight | Approx. 50 kg |
Related information
- FDSS Option 2: High Speed Acquisition Option [0.2 MB/PDF]
- FDSS Option 1: 1536-Well Format Option with Disposable Tips and Pin Tool Head [0.2 MB/PDF]
- FDSS Application Note No.27 Kinetic Luminescent Measurement of ROS (Reactive Oxygen Species) production with ABEL Cell activation ROS kit with PHOLASIN on the FDSS/μCELL platform [0.2 MB/PDF]
- FDSS Application Note No.26 BRET2 assay using the FDSS/μCELL: monitoring cell surface-receptor internalization [0.1 MB/PDF]
- FDSS Application Note No.25 Identification of small molecule modulators of reactive oxygen release in TNF-α primed primary human neutrophils. [0.1 MB/PDF]
- FDSS Application Note No.24 Evaluation of membrane potential assay kit using the FDSS/μCELL imaging plate reader: Detection ion channel in nicotinic acetylcholine receptors (nAChRs) [0.1 MB/PDF]
- FDSS Application Note No.23 BRET1-assay using the FDSS/μCELL imaging plate reader [0.1 MB/PDF]
- FDSS Application Note No.22 High Speed Data Acquisition Option for FDSS series [0.4 MB/PDF]
- FDSS Application Note No.21 Cell-based fluorescent assay for selectivity evaluation between two Na+ channels subtypes [0.2 MB/PDF]
- FDSS Application Note No.20 New Tools for Drug Discovery: Multiplexing the monitoring of Ca2+ and cAMP signaling in primary cells using the FDSS/μCell. [0.4 MB/PDF]
- FDSS Application Note No.19 Fast kinetics enzyme assay High-throughput screening of prolyl isomerase inhibitors [0.1 MB/PDF]
- FDSS Application Note No.18 β-arrestin recruitment for GPCR Screening [0.1 MB/PDF]
- FDSS Application Note No.17 Use of Ca2+ oscillations in primary neuron culture: a new HTS model for neuronal diseases [0.3 MB/PDF]
- FDSS Application Note No.16 FluxORTM Differentially Identifies K+ Channel Antagonists Using FDSS6000 [0.1 MB/PDF]
- FDSS Application Note No.15 Exposing cells to a single pulse of light of user-defined wavelength and duration using the FDSS6000 [0.1 MB/PDF]
- FDSS Application Note No.14 Evaluation of a new intracellular calcium fluorescent reagent using the FDSS6000 [0.4 MB/PDF]
- FDSS Application Note No.13 Incorporating Cell Viability Assays into High Throughput Screening Using the FDSS6000 [0.1 MB/PDF]
- FDSS Application Note No.12 Fluorescence Quenching in Cell-Based Assays Can Be Dye Specific: A Case Study Using Both Fura-2 AM and Fluo-4 AM Calcium Reporter Dyes Using the FDSS6000 [0.1 MB/PDF]
- FDSS Application Note No.11 Detection and Modulation of Cardiomyocyte Calcium Oscillations Using the FDSS6000 [0.1 MB/PDF]
- FDSS Application Note No.10 Comparison of Orthogonal Technologies to Confirm Agonist Hits Generated Using the FDSS6000 [0.1 MB/PDF]
- FDSS Application Note No.9 Effect of Plate Mixing, Fluid Addition Height and Speed on Reducing Addition Artifacts and Negative Control Drift Using the FDSS6000 [0.1 MB/PDF]
- FDSS Application Note No.8 Comparison of No Wash Reagent Kits on the FDSS6000 [0.4 MB/PDF]
- FDSS Application Note No.7 Multiplexing Calcium Mobilization and Membrane Potential Assays Using the FDSS6000 [0.3 MB/PDF]
- FDSS Application Note No.6 Direct comparison of Fast response and Slow response Membrane potential dye using the FDSS6000 [0.1 MB/PDF]
- FDSS Application Note No.5 COX flash luminescence screening [0.1 MB/PDF]
- FDSS Application Note No.4 FDSS Cell dispensing unit for Aequorin Flash luminescence [0.4 MB/PDF]
- FDSS Application Note No.3 FRET-based Voltage Sensor dyes for Drug Screening [0.1 MB/PDF]
- FDSS Application Note No.2 CoroNa Red as a potential probe for intracellular sodium imaging [0.1 MB/PDF]
- FDSS Application Note No.1 Chloride ion sensitive fluorescent dye for Drug Screening [0.1 MB/PDF]
