In-vitro toxicity assay
iPS derived cells drug discovery in FDSS
Recently, studies of iPS cells (induced pluripotent stem cells) have made a huge impact in the drug discovery field. Currently, human iPS cell (hiPSC) derived various specific types of cells such as cardiomyocytes and neural cells are now widely available commercially, and the screening of chemical compounds for drug discovery using these hiPSC-derived cells is possible. Screening using hiPSC-derived cells is expected to provide more effective and easy way to evaluate the pharmacological and toxic effects of test compounds in cell-based assays.
HAMAMATSU FDSS series are capable for the measurement and analysis of calcium transient in hiPSC-derived cardiomyocytes.
iPSC derived Cardiomyocyte for Safety & Vascular [0.8MB / PDF
iPSC derived Neurons [4.4MB / PDF
Both FDSS7000EX and FDSS/μCELL can have the “Heater”, “High Speed Recording” and “Waveform Analysis” option, needed for iPSC cardiomyocyte calcium transient. “Heater” function becomes more and more important when handling iPSC, for its cell status and condition during the assay, especially affects the beating rate of cardiomyocyte. Another key technology and function to monitor the calcium transient is the “High Speed Recording” ability. Maximum around 100Hz sampling rate, shortest around 10ms interval, reading is possible with our high sensitive high speed EM-CCD camera. We also have an option of “Waveform analysis” software, to analyze the number of peak, beating rate, amplitude, rising slope, falling slope PWD from the measured calcium transient data.
- Calcium oscillation in Cardiac cells
- iPSC derived cardiomyocytes for cardiac toxicity
- Pacing cardiomyocyte beating
Related scientific material
Please note that information in posters and presentations used in past conferences and symposiums may now be slightly different. As Hamamatsu are solely an equipment manufacturer, we are not responsible for measurement samples or data presented in these materials.
- Publications
-
Authors Title Source Stephane Bedut, Christine Seminatore Nole, Veronique Lamamy, Sarah Caignard, Jean Albert Boutin, Olivier Nosjean, Jean-Philippe Stephan, Francis Coge High-throughput drug profiling with voltage and calcium-sensitive fluorescent probes in human iPSC-derived cardiomyocytes American Journal of Physiology - Heart and Circulatory Physiology
Published 3 May 2016, Vol. no.
DOI:
10.1152/ajpheart.00793.2015Haoyu Zeng, Maria I. Roman, Edward Lis, Armando Lagrutta, Frederick Sannajust, SALAR, Safety & Exploratory Pharmacology Department, Merck Research Laboratories, West Point, PA 19486, USA Use of FDSS/μCell imaging platform for preclinical cardiac electrophysiology safety screening of compounds in human induced pluripotent stem cell-derived cardiomyocytes Received 29 February 2016
Revised 6 May 2016
Accepted 18 May 2016
Available online 21 May 2016
http://dx.doi.org/10.1016/j.vascn.2016.05.009Dinesh Puppala, Leon P. Collis, Sunny Z. Sun, Vinicius Bonato, Xian Chen, Blake Anson, Mathew Pletcher, Bernard Fermini and Sandra J. Engle Comparative Gene Expression Profiling in Human-Induced Pluripotent Stem Cell—Derived Cardiocytes and Human and Cynomolgus Heart Tissue Toxicol. Sci. (2013) 131 (1): 292-301.
First published online: Sept. 14, 2012
- Application Notes
-
Application Note
N°. 17FDSS Application Note No.17 Use of Ca2+ oscillations in primary neuron culture: a new HTS model for neuronal diseases [0.3MB/PDF] Application Note
N°. 22FDSS Application Note No.22 High Speed Acquisition for FDSS series [0.4MB/PDF]
- Additional Information
- Related Scientific Materials from Users Meetings in the past
